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Metal specificity of PA-Ntera
| Ion | Site |
Kd (μM) |
|
|---|---|---|---|
| wt | E80A | ||
| Mn2+ | High affinity | 0.3 | 5.2 |
| Low affinity | 6.5 | 46 | |
| Mg2+ | High affinity | 148 | |
| Low affinity | 4,000 | ||
Isothermal titration calorimetry experiments were performed on a VP-ITC calorimeter using protein solutions at a concentration of 60 μM and metal solution concentrations between 0.8 and 9 mM.
