FIG. 2.

Southern blot analysis of NIFV vector stocks. Purified DNAs from vector stocks of ΔφMscvF (Δφ), NIFV-MscvF (NIFV), D100V-MscvF (100), D157A-MscvF (157), and a pseudostock control (Ps) were cut with PstI and AgeI and analyzed by Southern blotting. DNAs were also digested with DpnI to remove transfected plasmid DNA. The positions of the linear DNA genome (linear), replication intermediates (R1 and R2), and 1-LTR circles (1-LTR) are shown. Diagrams of the predicted sizes (not to scale) of LTR-containing fragments are below the Southern blot. The position of the probe is denoted by the black bar.