TABLE 1.
Titers of NIFV vectors in HT-1080 cells
| Vector | No. of vector DNA genomes/ml | No. of transducing units/ml | No. of genomes/transducing unit | MFIa |
|||
|---|---|---|---|---|---|---|---|
| Day 1 |
Day 2 |
||||||
| Raw | Relative | Raw | Relative | ||||
| ΔφMscvF | 3.77 × 108 | 5.97 × 106 | 63 | 113 | 1.00 | 475 | 1.00 |
| NIFV-MscvF | 6.40 × 108 | 1.35 × 107 | 47 | 65 | 0.58 | 116 | 0.24 |
| D100V-MscvF | 3.72 × 108 | 8.45 × 106 | 44 | 73 | 0.65 | 125 | 0.26 |
| D157A-MscvF | 3.95 × 108 | 6.28 × 106 | 63 | 61 | 0.54 | 107 | 0.23 |
a
MFI determined by gating on GFP+ cells with a window containing <0.1% GFP+ cells in uninfected cultures.