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. 2010 Jun 30;84(18):9047–9058. doi: 10.1128/JVI.00698-10

FIG. 5.

FIG. 5.

The EV109 5′ UTR region reveals evidence of an ancestral recombination event. (A) Phylogenetic tree constructed from 5′ UTR regions of 74 annotated human enteroviruses. A black arrow denotes EV109. Also highlighted are CAV19 (red arrow), EV92 (blue arrow), and EV68 (yellow arrow), which are used in the subsequent bootscanning analysis. ClustalW and MEGA were used for alignments and tree construction, respectively, using the neighbor-joining method and 500 bootstrap replicates. (B) Bootscanning analysis of EV109. Bootscanning analysis was performed with other serotypes of HEV-A (EV92), HEV-C (CAV19), and HEV-D (EV68) using a word size of 400 and step size of 20. (C) Sequence alignment depicting the 5′ UTR-VP4 junction site. The EV109 sequence is compared to representative members of HEV-A and HEV-C.