TABLE 2.
Primers used for EV109 sequencing and genome recovery
| Primer name | Length (bases) | Start position (nt) | End position (nt) | Sequence (5′-3′) |
|---|---|---|---|---|
| EV109 5′ RACE 268R | 19 | 268 | 250 | ATGGTGGTACTAGGCTTCC |
| EV109 5′ RACE 274R | 20 | 274 | 255 | CAATCCATGGTGGTACTAGG |
| EV109 5′ RACE 333R | 17 | 333 | 317 | TATTCGGAGCCTCATCG |
| EV109 5′ UTR 492F | 19 | 492 | 510 | CAAACCAGCGTCTGGTAGG |
| EV109 VP4 849R | 21 | 849 | 829 | AATCATCACATCCTTAACTGG |
| EV109 VP2 942F | 20 | 942 | 961 | TACTCAAGAAGCAGCTAACG |
| EV109 VP3 1870F | 20 | 1870 | 1889 | GGGTATGCCTCCATTGTGTTG |
| EV109 VP3 1889R | 21 | 1889 | 1869 | AACACAATGGAGGCATACCC |
| EV109 VP1 2529F | 23 | 2529 | 2551 | GGAGACTGGAGCAACTAGTAAAG |
| EV109 VP1 2769R | 24 | 2769 | 2746 | GGTGAACATTTCCAATTTCCTACG |
| EV109 VP1 3030R | 21 | 3030 | 3010 | GGAAAAGCCGTCATAGAAGTG |
| EV109 2A deg 3662F | 19 | 3662 | 3680 | TCACTGCHGGKGGWGAAGG |
| EV109 2A deg 3688R | 17 | 3688 | 3704 | GCHTTTGCWGACATYAG |
| EV109 2C 4255F | 20 | 4255 | 4274 | ACACTGTTCAACAACATTCG |
| EV109 2C 4307F | 18 | 4307 | 4324 | CCTTGTATGCCAGCGAGG |
| EV109 2C 4324R | 18 | 4324 | 4307 | CCTCGCTGGCATACAAGG |
| EV109 2C 4513F | 17 | 4513 | 4529 | CCCTCTCACTTTGATGG |
| EV109 2C 4916R | 19 | 4916 | 4898 | TTGTCTACAAATTGAATGG |
| EV109 3C deg 5362F | 20 | 5362 | 5381 | GAYTAYGCAGTKGCAATGGC |
| EV109 3C 5452R | 18 | 5452 | 5435 | CTACCCGGTCATAAATGC |
| EV109 3D 5932R | 20 | 5932 | 5913 | CGTCTTTGCTGGGTCTCATC |
| EV109 3D 6286F | 20 | 6286 | 6305 | ATTCTGAACAAAGAAACACG |
| EV109 3′ RACE 7014F | 20 | 7014 | 7033 | GTTCTTGAAACGGTTTTTCC |
| EV109 3′ RACE 7044F | 20 | 7044 | 7063 | ACAATTCCCATACCTAGTGC |
| EV109 3D 7090R | 25 | 7090 | 7066 | CATATTTCTGACATTAGCATAACTGG |