TABLE 2.
Primers used for cloning expression vectors
| Construct name | Orientationd | Sequence (5′ to 3′)a |
|---|---|---|
| ICS-1 | FWD | ggtaccTGACTCGGCAGCCACTGTA |
| REV | ggatccTTTGTTCTTCAGTTTACCCTTCGGTTT | |
| ICS-2 | FWD | ggtaccGGGAATTTTTCAAAGATTCCATAATC |
| REV | ggatccAAGTCGGAAAATTCCTTATCACA | |
| ICS-3 | FWD | ggtaccAAACCGAAGGGTAAACTGAAGAACAAA |
| REV | ggatccATGATCTTCTCTCCTGCTTGGAAT | |
| ICS-4 | FWD | ggtaccGGACTGGCTTCCCTGGT |
| REV | ggatccCTTTTCCAAGAGTTCCTCGATGT | |
| ICS-5 | FWD | ggtaccTCGTCGAGCTCTTTGTCGTCCT |
| REV | ggatccAAAACGGCACAGATAACGTGAAA | |
| ICS-6 | FWD | ggtaccATGGTCGGACATCGAGGAACT |
| REV | ggatccTAATGACAGCCGCAAAATAGATCG | |
| ICS-7 | FWD | ggtaccGTACAGTTGTATAGCAGCACACG |
| REV | ggatccTACGAGTCGCGGATGATGTTA | |
| ICS-8 | FWD | ggtaccTTCACGTTATCTGTGCCGTTTTGC |
| REV | ggatccACCGTCCTCTGTCCGGATCTAC | |
| ICS-9 | FWD | ggtaccGGTGTACGTCGCTCTACATAGGAG |
| REV | ggatccTCTCGTCTCGTCCACCATTT | |
| ICS-10 | FWD | ggtaccTCCTCGTCGAGCTCTTTGTC |
| REV | ggatccTACGAGTCGCGGATGATGTTA | |
| ICS-11 | FWD | ggtaccTAAGAACCTGAGCACGCCGC |
| REV | ggatccTCTCGTCTCGTCCACCATTT | |
| ICS-12 | FWD | ggtaccGGGCTGGAGAATCTCTCGAA |
| REV | ggatccTCTCGTCTCGTCCACCATTT | |
| ICS-13 | FWD | ggtaccGGACTTGGACGTTGGAAATAAAT |
| REV | ggatccTCTCTCTTTTGTACAGCACTCGC | |
| ICS-14 | FWD | ggtaccGTCATTATCCTGAGAAGTGCCG |
| REV | ggatccTCTCTCTTTTGTACAGCACTCGC | |
| ICS7-RC | FWD | ggtaccTACGAGTCGCGGATGATGTTA |
| REV | ggatccGTACAGTTGTATAGCAGCACACG | |
| ICS-7A | FWD | ggtaccGTACAGTTGTATAGCAGCACACG |
| REV | ggatccTAATGACAGCCGCAAAATAGATCG | |
| ICS-7B | FWD | ggtaccGTACAGTTGTATAGCAGCACACG |
| REV | ggatccATTTCCACCGTGTCGAAGC | |
| ICS-7C | FWD | ggtaccTGAGTCGCATTCACCGGTTCT |
| REV | ggatccTACGAGTCGCGGATGATGTTA | |
| ICS-7D | FWD | ggtaccATCTATTTTGCGGCTGTCATTATCC |
| REV | ggatccTACGAGTCGCGGATGATGTTA | |
| KSHV-IRES | FWD | ggtaccTTGGACAGACTCCTACTTATAAAGC |
| REV | ggatccTTGTATATGTGAAGGCACCGAT | |
| KSHV-IRES-RC | FWD | ggtaccTTGTATATGTGAAGGCACCGATGT |
| REV | ggatccTTGGACAGACTCCTACTTATAAAGCAGG | |
| KSHV-IRES-pGL3 | FWD | ggtaccTTGGACAGACTCCTACTTATAAAGC |
| REV | aagcttTTGTATATGTGAAGGCACCGATGTG | |
| ICS-4-pGL3 | FWD | ggtaccGGACTGGCTTCCCTGGT |
| REV | aagcttGCCTTTTCCAAGAGTTCCTCGATGT | |
| ICS-7-pGL3 | FWD | ggtaccGTACAGTTGTATAGCAGCACACG |
| REV | aagcttTACGAGTCGCGGATGATGTTA | |
| ICS-7-RC-pGL3 | FWD | ggtaccTACGAGTCGCGGATGATGTTA |
| REV | aagcttTACGAGTCGCGGATGATGTTA | |
| FL | FWD | gtttaaacTTACACGGCGATCTTTCCGC |
| REV | tctagaATGGAAGACGCCAAAAACATAAAGAAAGGC | |
| FL-RC | FWD | tctagaTTGGCCTTTATGAGGATCTCTCTGATTTTTCTTG |
| REV | gtttaaacATGGAAGACGCCAAAAACATAAAGAAAGGC | |
| FIX-3.6 | FWD | gatatcGCAGTAGCGATGGGTTGCGACG |
| FWD | tctagaGCAGTAGCGATGGGTTGCGACG | |
| FIX-2.7 | FWD | gatatcCGGAGCTTGAGATTCCAAGCAGG |
| FWD | tctagaCGGAGCTTGAGATTCCAAGCAGG | |
| FIX-1.4 | FWD | gatatcGAGACATGCTCCACGATCTATTTT |
| FWD | tctagaGAGACATGCTCCACGATCTATTTT | |
| FIX-T-3′b | REV | gtttaaacTTACTGACTCATGTGAAAAGTGTGCTTTTTATTAACAGAGCAGAGGG |
| REV | gcgatcgcCATGTGAAAAGTGTGCTTTTTATTAACAGAGCAGAG | |
| UL133 | FWD | ATGGGTTGCGACGTGCACG |
| UL134 | FWD | ATGGCCAGGACCAGGG |
| UL135 | FWD | ATGTCCGTACACCGGCC |
| UL136 | FWD | ATGTCAGTCAAGGGCGTGGAG |
| UL137 | FWD | ATGGCCACGATCAGCACGAG |
| ORF-Mycc | REV | gatatcGCTCACAGATCCTCTTCTGAGATGAGTT |
a
Enzyme sites are in lowercase.
b
Used for all three cDNAs.
c
Used for all ORFs.
d
FWD, forward; REV, reverse.