FIG. 3.
The core complex is formed at the LD and ER. (a) The LD fraction and WCL were collected from JFH1E2FL RNA-transfected HuH-7 cells on day 5 posttransfection. (upper panel) Immunoblot analysis of the LD marker ADRP and the ER marker calnexin in the LD fraction; (lower panel) immunoblot analysis of the core protein in the LD fraction treated or not treated with 50 mM DTT. (b) Immunoblot analysis of the core protein in the MMF and WCL collected from JFH1E2FL-producing HuH-7 cells on day 5 posttransfection in the presence or absence of 5% β-ME. Data are representative of those from three independent experiments.