FIG. 4.

The core complex consists of a core dimer. (a) Schematic of wild-type, FLAG-tagged (FLAG-core), and Myc-tagged (Myc-core) core proteins. (b) Immunoblot analysis of the core protein in the MMF collected from HuH-7 cells transfected with combinations of pcDNA3 (vector) and/or core expression plasmids (e.g., encoding core^WT, FLAG-core, and Myc-core), as indicated. The experiment was performed under nonreducing conditions. The lower bands represent core monomer (marked on the right with a brace). White arrowheads, bands corresponding to dbd-core; black arrowheads, positions of the intermediately sized core complex formed by core^WT and the tagged core. Data are representative of those from three independent experiments.