FIG. 7.
JFH1C128A core inhibits JFH1E2FL particle assembly. A competitive inhibitory assay was performed with JFH1E2FL (WT) and JFH1C128A (C128A). (a) Real-time qRT-PCR analysis of the HCV RNA titer in HuH-7 cell culture medium 3 days after the cells were transfected with the indicated ratio of WT and C128A RNA. (b) The infectivity of culture medium collected from HuH-7 cells that had been transfected with the indicated ratio of WT and C128A RNA was analyzed as described in Materials and Methods. Data represent the means ± standard deviations from three independent experiments (a) or are representative of those from three independent experiments (b).