FIG. 2.

Characterization of T36 capsids by sucrose density gradient ultracentrifugation (a) and SDS-polyacrylamide gel electrophoresis (b). T36 capsids were prepared as described in Materials and Methods, beginning with HSV-1 released by TNE treatment of Vero cells infected for 18 h. Note that during sucrose density gradient centrifugation, T36 capsids migrated more rapidly than nuclear B capsids (left gradient) but more slowly than HSV-1 (right). SDS-polyacrylamide gel electrophoresis was carried out with material harvested from the starred bands in panel a. Note that the protein composition of T36 capsids (middle gel) was similar to that of nuclear C capsids (left gel), except that T36 capsids contained two additional protein species, UL36 and UL37 (asterisks).