FIG. 7.

Replication of the GNA/HHA-sensitive parental SIVmac239 and the GNA/HHA-resistant SIVN2 in rhesus macaque peripheral blood mononuclear cells (PBMCs). (A) Replication of SIVmac239 and SIVN2 in rhesus macaque PBMCs in the absence of lectin. (B) Replication of SIVmac239 and SIVN2 in rhesus macaque PBMCs in the presence of 1 mg/ml GNA and 0.5 mg/ml HHA. Rhesus macaque PBMCs were infected with HEK293T cell-produced SIVmac239 or SIVN2 that was normalized to contain equal amounts of p27 capsid protein. One day after infection, the cells were washed prior to being resuspended in medium with no lectin or with medium that contained 1 mg/ml GNA and 0.5 mg/ml HHA. SIV capsid protein (p27) that was released into the culture medium was measured on the days indicated to follow virus production.