TABLE 1.
Variability of field-isolated and laboratory-propagated FBNSV strains
| Genome component | FBNSV-[ET:Hol;1997] |
FBNSV-[ET:Hol;JKI-2000] |
FBNSV-[ET:Hol;ISV-T1] |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Length (nt) | No. of: |
Mutation frequency (×10−4) | Length (nt) | No. of: |
Mutation frequency (×10−4) | Length (nt) | No. of: |
Mutation frequency (×10−4) | ||||
| Number sequenced clones | Variable sitesa | Number sequenced clones | Variable sites | Number sequenced clones | Variable sites | |||||||
| DNA-R | 1,003 | 20 | 8 | 3.99 | 1,003 | 22 | 8 | 3.63 | 1,003 | 20 | 4 | 2.00 |
| DNA-S | 992 | 26 | 12 | 4.65 | 992 | 29 | 13 | 4.52 | 992 | 20 | 5 | 2.52 |
| DNA-C | 994 | 20 | 20 | 10.06 | 994 | 20 | 5 | 2.52 | 994 | 20 | 3 | 1.51 |
| DNA-M | 979 | 20 | 18 | 9.41 | 980 | 27 | 19 | 7.18 | 980 | 20 | 4 | 2.04 |
| DNA-N | 980 | 20 | 32 | 16.33 | 981 | 40 | 18 | 4.59 | 981 | 42 | 4 | 0.97 |
| DNA-U1 | 985 | 39 | 16 | 4.18 | 986 | 29 | 24 | 8.40 | 986 | 21 | 1 | 0.48 |
| DNA-U2 | 983 | 22 | 18 | 8.35 | 984 | 26 | 16 | 6.26 | 984 | 20 | 7 | 3.56 |
| DNA-U4 | 987 | 20 | 19 | 9.63 | 987 | 39 | 19 | 4.94 | 987 | 43 | 11 | 2.59 |
| Entire genome (correctedb) | 7,903 | 187 | 143 | 7.77 (7.52) | 7,907 | 232 | 122 | 5.33 (5.07) | 7,907 | 206 | 39 | 1.92 (1.66) |
| Total no. of nt sequenced | 184,000 | 229,049 | 203,392 | |||||||||
a
Variable sites include bases changes, indels, and recombination events.
b
The experimental error frequency of our amplification-cloning system of 2.53 × 10−05 was subtracted from the observed mutation frequency to yield the corrected mutation frequency.