TABLE 3.
Mutant spectra of field-isolated and laboratory-propagated FBNSV strains
| Sequence alteration | FBNSV-[ET:Hol;1997] |
FBNSV-[ET:Hol;JKI-2000] |
FBNSV-[ET:Hol;ISV-T1] |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No. observeda | No. expected | χ2 | P value | No. observed | No. expected | χ2 | P value | No. observed | No. expected | χ2 | P value | |
| Transitions | ||||||||||||
| Total | 86 | 43.67 | 61.5611 | 4.29 × 10−15 | 62 | 38 | 23.5820 | 1.20 × 10−6 | 21 | 11.33 | 12.3689 | 0.0004 |
| A → G | 11 (+7) | 12.72 | 0.2576-2.4273 | 0.6118-0.1192 | 10 | 10.93 | 0.0873 | 0.7677 | 5 | 3.29 | 0.9851 | 0.3209 |
| G → A | 16 (+7) | 9.58 | 4.6409-20.2801 | 0.0312-6.69 × 10−6 | 14 | 8.28 | 4.2718 | 0.0387 | 4 | 2.49 | 0.9879 | 0.3203 |
| C → T | 22 (+8) | 7.34 | 31.0509-74.1723 | 2.51 × 10−8-7.16 × 10−18 | 9 | 6.40 | 1.1234 | 0.2892 | 4 | 1.92 | 2.3765 | 0.1232 |
| T → C | 22 (+8) | 14.03 | 5.0704-20.3583 | 0.0243-6.42 × 10−6 | 29 | 12.06 | 26.6105 | 2.49 × 10−7 | 8 | 3.63 | 5.8885 | 0.0152 |
| Transversions | ||||||||||||
| Total | 45 | 87.33 | 61.5611 | 4.29 × 10−15 | 51 | 75 | 23.5820 | 1.20 × 10−6 | 13 | 22.66 | 12.3689 | 0.0004 |
| A → C | 4 (+2) | 12.72 | 6.6208-3.9321 | 0.0101-0.0474 | 7 | 10.93 | 1.5633 | 0.2112 | 3 | 3.29 | 0.0282 | 0.8667 |
| C → A | 9 (+2) | 7.34 | 0.3998-1.9386 | 0.5272-0.1638 | 3 | 6.40 | 1.9119 | 0.1668 | 0 | 1.92 | 2.0390 | 0.1533 |
| A → T | 4 (+1) | 12.72 | 6.6208-5.1894 | 0.0101-0.0227 | 8 | 10.93 | 0.8687 | 0.3513 | 2 | 3.29 | 0.5595 | 0.4545 |
| T → A | 6 (+1) | 14.03 | 5.1473-3.9451 | 0.0233-0.0470 | 12 | 12.06 | 0.0004 | 0.9840 | 2 | 3.63 | 0.8198 | 0.3652 |
| C → G | 3 | 7.34 | 2.7149 | 0.0994 | 2 | 6.40 | 3.2034 | 0.0735 | 0 | 1.92 | 2.0390 | 0.1533 |
| G → C | 2 | 9.58 | 6.4712 | 0.0110 | 4 | 8.28 | 2.3843 | 0.1226 | 0 | 2.49 | 2.6872 | 0.1012 |
| G → T | 7 (+3) | 9.58 | 0.7499-0.0198 | 0.3865-0.8880 | 9 | 8.28 | 0.0683 | 0.7938 | 4 | 2.49 | 0.9879 | 0.3203 |
| T → G | 4 (+3) | 14.03 | 8.0306-3.9451 | 0.0046-0.0470 | 6 | 12.06 | 3.4144 | 0.0646 | 2 | 3.63 | 0.8198 | 0.3652 |
| Ts/Tvb | 1.91 | 0.5 | 1.22 | 0.5 | 1.62 | 0.5 | ||||||
| Deletions | 6 | 0 | 1 | |||||||||
| Insertions | 3 | 3 | 2 | |||||||||
| Recombination events | 3 | 6 | 2 | |||||||||
a
Numbers in parentheses refer to potential additional mutations resulting from 50% ± 10% ambiguities of the consensus (reference) base and the respective change. Note that these numbers have to be identical for reciprocal changes (e.g., A → G and G → A, etc.), and they are counted only once for the total number of transitions or transversions.
b
Ts/Tv, transition/transversion ratio.