TABLE 3.
Comparison of the binding interaction between specific regions of the 3′ UTR RNA and NS5A(ΔAH) and NS5B proteins constructs as evaluated by filter binding assaya
| Genotype 1b (J4) protein and RNA region | Kd, nM (mean ± SE) | Endpoint, % (mean ± SE) |
|---|---|---|
| NS5A(ΔAH) | ||
| 3′ UTR | 56 ± 1.7 | 72 ± 3.8 |
| Poly(U/UC) | 44 ± 4.0 | 39 ± 1.9 |
| X region | 123 ± 5.3 | 16 ± 2.2 |
| NS5B | ||
| 3′ UTR | 25 ± 1.6 | 89 ± 1.8 |
| Poly(U/UC) | 75 ± 2.1 | 9.0 ± 2.9 |
| X region | 33 ± 1.2 | 54 ± 1.7 |
a
The binding affinity and percentage of the total RNA bound to the NS5A and NS5B proteins are shown for experiments conducted with proteins and RNA of genotype 1b.