FIG. 6.

HTNV inhibits poly(I:C)-mediated induction of IFN-β and IFN-λ1 in A549 cells. Infected (MOI of 5) and noninfected cells were stimulated with poly(I:C) (10 μg/ml) 48 h after HTNV infection or incubated with only medium as a control. Induction of IFN-β and IFN-λ1 mRNA expression at 1.5, 3, and 6 h after poly(I:C) stimulation was determined by Q-PCR. The data were normalized using β-actin, and relative expression in treated compared to nontreated cells was calculated for infected and noninfected cells, respectively. Data are presented as percent inhibition of relative expression for HTNV-infected compared to noninfected cells. Error bars represent standard deviations of the means from one representative experiment.