FIG. 4.

Effects of QC and 9AA on EMCV, poliovirus RNA, and p-VSV mRNA accumulation. Ten micrograms of total RNA prepared from HeLa cells 5 h postinfection (p.i.) with EMCV (MOI of 2 CCID₅₀/cell) (A), 3 h p.i. with poliovirus (MOI of 2 CCID₅₀/cell) (B), and 5 h p.i. VSV (C) was analyzed by Northern blotting with an EMCV IRES-specific probe, a poliovirus protein 3C-specific probe, and a p-VSV mRNA-specific probe, respectively. A human GAPDH probe was used as a control. Guanidine hydrochloride (GndCl) (final concentration of 1 mM) was used as a positive control of antiviral drug action.