FIG. 3.

PS-341 impairs viral propagation of different IAV strains in different cell types. A549 (A, B, and F), U937 (C), MDCK II (D), HEK293 (E), HUVEC (G), or HBEpC (H) cells were pretreated for 1 h with 50 nM PS-341 or solvent or left untreated. Then cells were infected with different human and avian IAV strains (FPV MOI of 0.001, PR8 MOI of 0.05, and KAN-1 MOI of 0.01). Subsequent to virus inoculation cells were posttreated with 50 nM PS-341 or solvent or left untreated. After the indicated times p.i. supernatants were obtained and progeny virus titers were determined by standard plaque assay. Arrow bars represent standard deviations of three independent experiments.