FIG. 6.

HPV16 E7 coexpression does not affect E6-induced S6K T389 phosphorylation or cap-dependent translation. (A) Western blot analysis of S6K T389 phosphorylation in HFK populations with stable expression of HPV16 E6 or HPV16 E6/E7 or control vector (LX)-transduced HFKs. An actin blot is shown as a loading control. (B) U2OS cells were transiently transfected with pFR_CrPV_xb and human β-actin-promoter-driven expression vectors for HPV16 E6, E7, E6/E7, the entire HPV16 early coding region (ER), or empty vector as a control and processed for Renilla and firefly luciferase assays at 48 h posttransfection. Firefly and Renilla luciferase activities were normalized to control vector-transfected cells and are presented as fold changes of normalized firefly relative to normalized Renilla luciferase activity. The bar represents the average and standard deviation of four experiments, each performed in triplicate; asterisks indicate statistical significance (P ≤ 0.0013).