FIG. 9.

Comparison of intracellular and extracellular accumulation of DENV and WNV NS1 by pulse-chase analysis. (A) BHK21 cells were infected with SINV-DENV-NS1 or SINV-WNV-NS1, labeled with [³⁵S]cysteine-methionine for 20 min, chased for the indicated times, and immunoprecipitated with cross-reactive 9NS1 MAb-Sepharose. (B) ³⁵S-labeled supernatants from mock-infected cells (M) or cells infected with SINV (Vector) or SINV expressing wild-type or chimeric (DS WNV, D121W, D87W, and D35W) NS1. Cells were infected for 12 h, pulsed with ³⁵S for 20 min, and chased for 3 h. Supernatants were harvested, immunoprecipitated with 9NS1 MAb-Sepharose, and electrophoresed. The data in this figure are representative of two to four independent experiments per panel.