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. 2010 Jul 14;48(9):3366–3369. doi: 10.1128/JCM.00992-10

FIG. 3.

FIG. 3.

Comparison of RD-114 virus and RD-114-related viruses. (A) Comparison of nucleotide sequences of the partial gag-pol gene of RD-114 virus (clone pSc3c) and RD-114-related viruses derived from CRFK cells and vaccine A. The first nucleotide for the initiation codon of the Gag-Pol precursor polyprotein of pCRT1 is defined as nucleotide position +1. Small letters and dashes indicate nucleotides and deletions, respectively. Capital letters in parentheses indicate amino acids. (B) Comparison of nucleotides of the U3 region of the LTR of RD-114 virus (clone pSc3c) and RD-114-related viruses derived from CRFK cells and vaccine A. (C) Comparison of the sizes of the entire LTRs of RD-114 virus and RD-114-related viruses. PCR for amplification of the entire LTR was conducted using genomic DNA of TE671/CRsup (lane 1) and TE671(LacZ)/VA (lane 2) cells and mock-infected TE671 (lane 5) and TE671(LacZ) (lane 6) cells. As positive controls, pSc3c (lane 3) and pCRT1 (lane 4) were used. Lane M, 100-bp-ladder molecular weight marker. (D) Long-range PCR used to clone the 5′ and 3′ halves of RD-114-related virus derived from vaccine A. The 5′ (lanes 1 and 3) and 3′ (lanes 2 and 4) halves of the RD-114-related virus were amplified by PCR from TE671(LacZ)/VA cells (lanes 1 and 2) and TE671(LacZ) cells (lanes 3 and 4). Lane M, 1-kb-ladder molecular weight marker.