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Multiplex nested PCR optimization. Lane M, 100-bp DNA ladder; lane 1, negative control; lane 2, positive M. tuberculosis DNA fragment; lane 3, positive P. jirovecii DNA fragment; lane 4, multiplex nested PCR with the fragments of the two species M. tuberculosis (141 bp) and P. jirovecii DNA (260 bp) together. The numbers on the left and right of the gel are molecular sizes (in base pairs).
