TABLE 4.
Results of multiplex nested PCR compared with those of single nested PCR for P. jirovecii detection
| Result by multiplex nested PCR | No. (%) of samples with the following single nested PCR resulta: |
||
|---|---|---|---|
| Positive | Negative | Total | |
| Positive | 35 (36.84) | 0 (0.00) | 35 (36.84) |
| Negative | 8 (8.42) | 52 (54.74) | 60 (63.16) |
| Total | 43 (45.26) | 52 (54.74) | 95 (100.00) |
a
By use of gold standard 2 for the single nested PCR. Sensitivity, 81.40% (95% confidence interval, 66.6 to 91.6); specificity, 100%; PPV, 100%; NPV, 86.67%.