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. 2010 Jul 28;48(9):3236–3243. doi: 10.1128/JCM.01019-10

TABLE 5.

Results of precision testing for C. trachomatis

Panel membera No. testedb No. positive Mean delta cycle SDc
Within-run component Between-run component Between-lot component Between-site component Total
1 150 150 14.78 0.300 0.194 0.066 0.137 0.388
2 149 149 15.15 0.385 0.139 0.285 0.000 0.499
3 149 149 3.12 0.591 0.241 0.000 0.047 0.640
4 150 150 8.89 0.385 0.156 0.169 0.162 0.477
5 148 0
6 148 148 16.88 0.167 0.207 0.149 0.215 0.373
7 150 0
8 149 1 0.67
9 148 103 1.09 0.637 0.000 0.192 0.000 0.665
a

C. trachomatis concentrations were targeted approximately to 4,500 IFU/assay in members 1, 2, and 6 and to 45 IFU/assay in member 4. The concentration for member 3 was targeted approximately to 0.75 IFU/assay and that for member 9 to 0.2 IFU/assay, both below the claimed assay limit of detection. Members 5, 7, and 8 did not contain any C. trachomatis organisms.

b

Invalid replicates were excluded from the analysis.

c

The SD is based on positive replicates only. For member 9, analysis of all replicates with a cycle number (n = 133), including those beyond the assay cutoff, resulted in a total SD of 0.966. The total variability contains within-run, between-run, between-lot, and between-site variability.