TABLE 2.
Distinctive biochemical characteristics of C. xerosis, C. freneyi, C. amycolatum, and C. hansenii
| Characteristica | % Positive reactions |
|||
|---|---|---|---|---|
| C. xerosis (n = 20) | C. freneyi (n = 7) | C. amycolatum (n = 9) | C. hansenii (n = 1) | |
| Fermentation of glucose at 42°C | 0 | 100 | 100 | 0 |
| Growth at 20°C | 100 | 100 | 0 | 100 |
| API Coryne | ||||
| Nitrate reduction | 90 | 71 | 44 | 0 |
| Fermentation of sucrose | 100 | 100 | 56 | 100 |
| Production of: | ||||
| Alkaline phosphatase | 100 | 100 | 100 | 0 |
| Pyrazinamidase | 100 | 100 | 89 | 100 |
| α-Glucosidase (pH 7.4) | 100 | 86 | 0 | 0 |
| API 50CH (fermentation of:) | ||||
| Glycerol | 17 | 0 | 100 | 100 |
| Erythritol | 5 | 0 | 0 | 0 |
| d-Arabinose | 5 | 0 | 0 | 0 |
| l-Arabinose | 5 | 0 | 11 | 0 |
| Adonitol | 5 | 0 | 0 | 0 |
| Galactose | 94 | 100 | 44 | 100 |
| d-Mannose | 84 | 83 | 100 | 100 |
| l-Sorbose | 0 | 16 | 0 | 0 |
| Rhamnose | 17 | 16 | 0 | 0 |
| Dulcitol | 0 | 16 | 0 | 0 |
| Sorbitol | 0 | 16 | 0 | 0 |
| α-Methyl-d-mannoside | 5 | 0 | 0 | 0 |
| α-Methyl-d-glucoside | 5 | 0 | 0 | 0 |
| N-Acetyl-glucosamine | 11 | 0 | 0 | 0 |
| Amygdaline | 27 | 16 | 11 | 0 |
| Arbutin | 17 | 33 | 0 | 0 |
| Salicin | 22 | 16 | 11 | 0 |
| Cellobiose | 11 | 33 | 33 | 0 |
| Melibiose | 5 | 0 | 22 | 0 |
| Trehalose | 99 | 100 | 56 | 100 |
| Inulin | 52 | 16 | 0 | 0 |
| d-Raffinose | 0 | 0 | 11 | 0 |
| Amidon | 22 | 33 | 11 | 0 |
| β-Gentiobiose | 27 | 16 | 0 | 0 |
| d-Turanose | 83 | 83 | 0 | 0 |
| d-Lyxose | 5 | 0 | 0 | 0 |
| d-Tagatose | 5 | 16 | 0 | 0 |
| d-Fucose | 11 | 0 | 0 | 0 |
| l-Fucose | 22 | 16 | 0 | 0 |
| d-Arabitol | 0 | 16 | 0 | 0 |
| l-Arabitol | 0 | 16 | 0 | 0 |
| Gluconate | 11 | 83 | 0 | 0 |
| 2-Ketogluconate | 5 | 16 | 0 | 0 |
| 5-Ketogluconate | 99 | 83 | 0 | 0 |
| API ZYM | ||||
| Esterase (C4) | 100 | 83 | 100 | 100 |
| Lipase (C14) | 61 | 66 | 89 | 0 |
| Leucine arylamidase | 99 | 100 | 89 | 100 |
| Acid phosphatase | 0 | 0 | 89 | 0 |
| Naphthol-AS-BI-phosphohydrolase | 61 | 16 | 67 | 0 |
a
Those tests that can be useful for the differentiation of C. xerosis strains from those of C. freneyi, C. amycolatum, and C. hansenii are in bold format.