TABLE 1.
Primers and annealing temperatures for the detection of B. henselae by PCR
| Target gene and assay (segment amplified) | Primer name | Primer sequence (5′-3′) | Annealing temp (°C) | Amplicon size (bp) | Reference(s) |
|---|---|---|---|---|---|
| htrA | |||||
| Assay I (nt 1209-1372) | 3+ (outer forward) | GTG CGT TAA TTA CCG ATC CA | 58.0 | 163 | 3, 5 |
| 4− (outer reverse) | CCA AAC TCC TAA GGT TAC TGT TTC | 58.0 | 5 | ||
| 1+ (inner forward) | GCT GGT ATC AAG GCA GGT G | 58.0 | 82 | 5 | |
| 2− (inner reverse) | GCA ATA CGC TTT GCT AGA TCAC | 58.0 | 5 | ||
| Assay II (nt 1250-1332) | 6+ (outer forward) | CCA GAT CAA CAT CCC TTG AAA | 58.0 | 182 | 5 |
| 5− (outer reverse) | GCG TAA CTT GTG CCA TCA GA | 58.0 | 5 | ||
| 7+ (inner forward) | TTT GCA ACG TTC GCA TAG ACT | 58.0 | 93 | 5 | |
| 8− (inner reverse) | CAG AAA TCA CAT GAT TAT TGG TCAC | 58.0 | 5 | ||
| gltA (nt 504-1178) | f1 (outer forward) | GGT CCC AAC TCT TGC CGC TAT G | 65.0 | 675 | 34 |
| r1 (outer reverse) | CAG CCG ACA CTG CGT GCT AAT G | 65.0 | 34 | ||
| f2 (inner forward) | ATG CCT AAA AAT GTT ACA AGA | 58.0 | 354 | 34 | |
| r2 (inner reverse) | CGT GCT AAT GCA AAA AGA AC | 58.0 | 34 |