TABLE 2.
Primers and annealing temperatures for the detection of FIV, FeLV, and FPV by PCR
| Assay/target gene (segment amplified) | Primer name | Primer sequence (5′-3′) | Annealing temp (°C) | Amplicon size (bp) | Source or reference(s) |
|---|---|---|---|---|---|
| FIV provirus/gag p26 (nt 1057-1639) | E 1+ (forward) | CCA CAA TAT GTA GCA CTT GAC C | 56.0 | 11 | |
| E 2− (outer reverse) | GGG TAC TTT CTG GCT TAA GGT G | 56.0 | 583 | 11 | |
| N 4− (inner reverse) | TAA TGG TCT GGG AGC ATC AG | 56.0 | 249 | This report | |
| FeLV provirus/U3 LTR (nt 2107-2290) | f1 (outer forward) | TTA CTC AAG TAT GTT CCC ATG | 55.0 | 184 | 18 |
| r1 (reverse) | AGG TCG AAC TCT GGT CAA CT | 55.0 | 18, 40 | ||
| f2 (inner forward) | CTT GAG GCC AAG AAC AGT TA | 52.7 | 104 | 18, 40 | |
| FPV/VP2 gene (nt 851-1022) | 1A+ (forward) | CAT TGG GCT TAC CAC CAT TT | 55.0 | 172 | This report |
| 2− (reverse) | GGT GCA CTA TAA CCA ACC TCA GC | 55.0 | 37 |