TABLE 5.
Comparison of identification results between sputum HMPRT-PCR and same culture-based rpoB PRA for 117 sputum samples, according to mycobacterial species
| Species | Strain score by HMPRT-PCR for the following scores obtained by culture-based rpoB PRAa: |
Sensitivity (%)b | |||||
|---|---|---|---|---|---|---|---|
| Trace | 1+ | 2+ | 3+ | 4+ | Total | ||
| M. intracellulare | 12 (8/0/4) | 11 (10/0/1) | 4 (4/0/0) | 4 (4/0/0) | 1 (1/0/0) | 32 (27/0/5) | 84.3 |
| M. avium | 13 (6/0/7) | 3 (0/0/3) | 4 (4/0/0) | 7 (6/0/1) | 3 (3/0/0) | 30 (19/0/11) | 63.3 |
| M. abscessus | 8 (4/2/2) | 12 (7/0/5) | 4 (4/0/0) | 6 (5/0/1) | 0 | 30 (20/2/8)c | 66.7 |
| M. tuberculosis | 3 (3/0/0) | 1 (1/0/0) | 3 (3/0/0) | 7 (7/0/0) | 7 (7/0/0) | 21 (21/0/0) | 100 |
| M. fortuitum | 0 | 0 | 0 | 0 | 1 (1/0/0) | 1 (1/0/0) | 100 |
| M. kansasii | 0 | 0 | 1 (1/0/0) | 0 | 0 | 1 (1/0/0) | 100 |
| M. szulgai | 0 | 1 (0/0/1) | 0 | 0 | 0 | 1 (0/0/1) | 0 |
| M. celatum | 0 | 0 | 0 | 1 (0/0/1) | 0 | 1 (0/0/1) | 0 |
| Total | 36 (21/2/13) | 28 (18/0/10) | 16 (16/0/0) | 25 (22/0/3) | 12 (12/0/0) | 117 (89/2/26) | |
| Sensitivity (%) | 58.3 | 64.3 | 100 | 88 | 100 | 76.1 | |
a
Data in parentheses represent the number of isolates for which the results of the two methods are in agreement/number of isolates for which the results are in disagreement/number of isolates not identified by HMPRT-PCR.
b
Calculated from the concordant results between the two assays, rpoB PRA and HMPRT-PCR.
c
Differentiation between M. abscessus (12 samples) and M. massiliense (8 samples) was possible by HMPRT-PCR.