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Probit analysis of the pandemic H1N1/09 influenza (Inf A/H1) and influenza A (Inf A/M2) virus RT-qPCR assays. Diamond datum points represent the observed proportion of positive test results of all replicates tested at a given RNA input concentration. The statistically predicted proportion of positive results (solid line) and 95% confidence intervals (broken lines) are indicated. LOD95, 95% limit of detection.
