TABLE 3.
Comparison of the analytical sensitivities of four RT-qPCR assays for the detection of influenza A/H1N1/09 virus in quadruple test series
| Assaya | Sensitivity (test 1/test 2/test 3/test 4) to A/Hamburg/4/2009 at a log10 dilution of: |
|||||
|---|---|---|---|---|---|---|
| 1 × 10−2 | 1 × 10−3 | 1 × 10−4 | 1 × 10−5 | 1 × 10−6 | 1 × 10−7 | |
| HA gene target | ||||||
| ROC | +/+/+/+ | +/+/+/+ | +/+/+/+ | +/+/+/+ | +/−/+/− | −/−/−/− |
| PAN | +/+/+/+ | +/+/+/+ | +/+/+/+ | +/+/+/+ | +/+/+/− | −/−/+/− |
| CDC-L | +/+/+/+ | +/+/+/+ | +/+/+/− | +/+/+/− | −/−/−/− | −/−/−/− |
| ART | +/+/+/+ | +/+/+/+ | +/+/+/+ | +/+/+/+ | +/+/+/− | −/−/−/− |
| MA gene target | ||||||
| ROC | +/+/+/+ | +/+/+/+ | +/+/+/+ | +/+/+/+ | +/−/−/− | −/−/−/− |
| PAN | +/+/+/+ | +/+/+/+ | +/+/+/+ | +/+/+/+ | +/−/−/− | −/−/−/− |
| CDC-L | +/+/+/+ | +/+/+/+ | +/+/+/+ | +/+/+/+ | +/+/+/+ | +/−/+/− |
| ART | +/+/+/+ | +/+/+/+ | +/+/+/+ | +/+/+/+ | +/−/+/− | +/−/+/− |
a
ROC, Roche RealTime Ready Influenza A/H1N1 Detection Set; PAN, assay described previously by Panning et al. 12; CDC-L, CDC-like rRT-PCR Swine Flu Panel (TIB Molbiol, Berlin, Germany); ART, Artus Infl./H1 LC/RG RT-PCR kit (Qiagen, Hilden, Germany).