TABLE 1.
Comparison of qualitative detection of adenovirus DNA in different samples using an in-house real-time qPCR assay and the Adenovirus R-Gene kita
Sample type | No. of samples giving: |
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---|---|---|---|---|---|---|
Concordant results |
Discordant results |
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Detectable | Nondetectable | Total | Detectable using in-house assay | Detectable using kit assay | Total | |
Cerebrospinal fluid (n = 22) | 1 | 20 | 21 | 1 | 0 | 1 |
Digestive biopsy specimen (n = 24) | 11 | 11 | 22 | 2 | 0 | 2 |
Whole blood (n = 78) | 55 | 19 | 74 | 2 | 2 | 4 |
Stools (n = 26) | 19 | 5 | 24 | 0 | 2 | 2 |
All samples (n = 150) | 86 | 55 | 141 | 5b | 4c | 9 |
The in-house real-time qPCR assay is that of Heim et al. (12).
The CT values for discrepant samples were 35.43, 35.53, 38.25, 40.63, and 33.62.
The CT values for discrepant samples were 42.92, 37.66, 40.68, and 38.96.