TABLE 1.
Comparison of qualitative detection of adenovirus DNA in different samples using an in-house real-time qPCR assay and the Adenovirus R-Gene kita
| Sample type | No. of samples giving: |
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|---|---|---|---|---|---|---|
| Concordant results |
Discordant results |
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| Detectable | Nondetectable | Total | Detectable using in-house assay | Detectable using kit assay | Total | |
| Cerebrospinal fluid (n = 22) | 1 | 20 | 21 | 1 | 0 | 1 |
| Digestive biopsy specimen (n = 24) | 11 | 11 | 22 | 2 | 0 | 2 |
| Whole blood (n = 78) | 55 | 19 | 74 | 2 | 2 | 4 |
| Stools (n = 26) | 19 | 5 | 24 | 0 | 2 | 2 |
| All samples (n = 150) | 86 | 55 | 141 | 5b | 4c | 9 |
a
The in-house real-time qPCR assay is that of Heim et al. (12).
b
The CT values for discrepant samples were 35.43, 35.53, 38.25, 40.63, and 33.62.
c
The CT values for discrepant samples were 42.92, 37.66, 40.68, and 38.96.