TABLE 2.
Intra- and interassay reproducibilities of the Adenovirus R-Gene kit using serial dilutions of an adenovirus serotype 2 strain isolated from a hematopoietic stem cell recipient
Dilution | Intra-assay results (n = 5) |
Interassay results (n = 6) |
||
---|---|---|---|---|
Range of CT values | Coefficient of variation (%) | Range of CT values | Coefficient of variation (%) | |
Undiluted (3.52 × 107) | 18.49-19.12 | 1.38 | 18.72-19.19 | 1.09 |
1:10 (3.52 × 106) | 22.09-22.67 | 0.96 | 22.34-23.03 | 1.15 |
1:102 (3.52 × 105) | 25.84-26.27 | 0.73 | 26.21-27.79 | 2.11 |
1:103 (3.52 × 104) | 29.00-29.57 | 0.89 | 29.57-30.74 | 1.71 |
1:104 (3.52 × 103) | 32.31-33.90 | 1.89 | 32.77-35.16 | 2.40 |
1:105 (3.52 × 102) | 35.07-37.10 | 2.24 | NRa | |
1:106 (3.52 × 101) | NDb | NR | ||
Negative control | ND | ND |
NR, not realized.
ND, nondetectable.