TABLE 2.
Identification of 52 blood culture bottle samples by PCR-HRMA and conventional blood culture methods
| Conventional culture identification (no. of isolates) | Grouping code of analysis subsets |
Signature code | PCR-HRMA identification (no. of isolates) | Analysis result for discordant samples (based on reculturing/sequencing) | ||
|---|---|---|---|---|---|---|
| V1 | V3 | V6 | ||||
| Concordant samples | ||||||
| S. aureus (10) | h | e | g | heg | S. aureus (10) | |
| CoNS (9) | n | e | a | nea | S. epidermidis (4) | |
| b | e | b | beb | S. haemolyticus (2) | ||
| d | a | e | dae | S. lugdunensis (1) | ||
| b | a | b | bab | S. hominis (1) | ||
| S. agalactiae (5) | m | c | c | mcc | S. agalactiae (5) | |
| MRSAa (3) | h | e | g | heg | S. aureus (3) | |
| P. aeruginosa (3) | f | c | l | fcl | P. aeruginosa (3) | |
| H. influenzae (3) | a | e | b | aeb | H. influenzae (3) | |
| E. coli (2) | a | g | d | agd | E. coli (2) | |
| K. pneumoniae (2) | e | i | d | eid | K. pneumoniae (2) | |
| M. morganii (2) | j | h | j | jhj | M. morganii (2) | |
| M. luteus (2) | g | i | h | gih | M. luteus (2) | |
| S. epidermidis (1) | n | e | a | nea | S. epidermidis (1) | |
| E. faecalis (1) | c | h | a | cha | E. faecalis (1) | |
| E. aerogenes (1) | c | b | e | cbe | E. aerogenes (1) | |
| Enterobacter sp. (1) | c | b | e | cbe | E. aerogenes (1) | |
| S. pyogenes (1) | m | a | d | mad | S. pyogenes (1) | |
| Discordant samples | ||||||
| F. varium (1) (unidentified)b | Staphylococcus xylosus | |||||
| CoNS (1) (unidentified)b | Mixed β Streptococcus species | |||||
| S. pyogenes (2) (polymicrobial)c | α Streptococcus species, Enterobacteriaceae species | |||||
| Viridans streptococci (2) (polymicrobial)c | α Streptococcus species, Enterobacteriaceae species | |||||
a
MRSA, methicillin-resistant S. aureus.
b
A single melt curve was generated for each analysis subset but was not associated with a pathogen within our reference organism library.
c
Multiple melt curve peaks indicated polymicrobial infection.