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. 2010 Jun 30;48(9):3176–3181. doi: 10.1128/JCM.00420-10

FIG. 5.

FIG. 5.

Optimization of skin test response-inducing defined antigen combinations. Skin test responses induced by the SICCT test (PPD-B − PPD-A) and a selection of protein- and peptide-based antigen combinations were determined for cattle naturally exposed to M. bovis (n = 13). Protein combinations of ESAT-6 (E6), CFP-10 (C10), MPB70, MPB83 (83), and/or Rv3615c were all tested at a concentration of 10 μg per constituent protein. The ESAT-6 and CFP-10 (E6/C10) peptide cocktail comprised 21 peptides at a concentration of 10 μg per peptide. Responses measured at 72 h for each animal are represented by open circles, and the horizontal line provides the mean (±SEM), with results expressed as the difference in skin thicknesses (mm) between the pre- and post-skin test readings. The statistical difference between responses induced by the indicated cocktails was determined by using ANOVA (*, P < 0.05).