FIG. 3.
Representative images of Live/Dead staining in P. acanthamoebae (A) and numbers of P. acanthamoebae infective progeny in cultures without amoebae (B). (A) The bacteria at 28 days after incubation were stained with a Live/Dead staining kit. Green, membrane integrity stable (possibly viable bacteria); red, dead bacteria. Magnification, ×400. (B) A bacterial solution of 100 μl containing approximately 107 to 108 AIU prepared with PAS was inoculated into the wells of a 24-well plate with (moist conditions) or without (dry conditions) 900 μl of PAS and incubated for up to 28 days at 15°C or 30°C in a normal atmosphere. The numbers of infective progeny as a marker of bacterial viability in the solution were determined using the AIU assay. The data represent the average AIU counts ± standard deviations. *, P < 0.05.