TABLE 1.

Bordetella isolates with positive or inconclusive RT-PCR results for BP3385^a

Species	Isolate or strain	CT	Hybridization to BP3385 probe/approx fragment size (kb)	Amplicon obtained with BP3385-1/BP3385-2	BP3385 allelic variant
B. pertussis	Tohama	26.2	+/4.7	+	1
B. bronchiseptica	RB50	ND	−	−
B. bronchiseptica	PV6	26.1	+/8.0	+	4
B. bronchiseptica	OSU-083	42.9	+/3.8	+	5
B. bronchiseptica	OSU-189	35.0	−	ND
B. bronchiseptica	OSU-553	34.6	−	ND
B. bronchiseptica	OSU-585	32.9	−	ND
B. bronchiseptica	ISU-CA90 BB1187	37.8	−	ND
B. bronchiseptica	ISU-CA90 BB135	30.1	−	ND
B. bronchiseptica	ISU-WI91 BB062/T5	40.8	+/3.8	+	5
B. bronchiseptica	ISU-WI91 BB064/T3	40.9	+/3.8	+	5
B. hinzii	ATCC 51783	36.8	+/7.9	+	3
B. hinzii	4134	35.5	+/7.9	+	3
B. hinzii	4135	36.0	+/11.2	+	2
B. hinzii	4140	32.3	−	ND
B. hinzii	4159	35.5	+/7.9	+	3
B. hinzii	4161	35.3	+/7.9	+	3
B. hinzii	4449	33.3	−	ND
B. hinzii	4595	35.2	+/7.9	+	3
B. hinzii	OH87 BAL006II	34.8	+/11.2	+	2
B. hinzii	OH87 BAL007II	44.2	−	ND
B. hinzii	OH87 BAL028II	34.7	+/7.9	+	3
B. hinzii	OH87 BAL030II	35.2	+/7.9	+	3
B. hinzii	CA90 BAL33	35.6	+/7.9	+	3
B. hinzii	CA90 BAL1384	38.9	−	ND
B. hinzii	BAL168II	35.6	+/7.9	+	3
B. hinzii	F-1	33.6	−	−
B. hinzii	1277	34.4	−	−
B. hinzii	DBL191	34.2	−	ND

^aND, not determined; +, present; −, absent.