FIG. 3.

Effect of Anx2 depletion on the intracellular localization of Gag-GFP and endosomal markers. Stably transduced COS-1 cells (Scr and Anx2) were transfected with Gag-GFP and then seeded onto glass coverslips. Cells were fixed, permeabilized and stained with DAPI and anti-EEA1 antibody (A), anti-CD63 antibody (B), or anti-Anx2 antibody (C). The top panels in panels A and B are control cells, and the bottom panels are Anx2-depleted cells. In panel C, the panel on the left shows control cells and the panel on the right shows Anx2-depleted cells.