TABLE 1.
Viruses used in this study
| Virus | Transgene type | Promotera | ER signal peptideb | pIX fusion proteinc | NLSd |
|---|---|---|---|---|---|
| AdRP2330 | pIX | − | − | ||
| AdAVH6 | GFP | pIX | − | − | |
| AdRP2218 | mSEAP | pIX | − | − | |
| AdRP2310 | pIX | − | GFP | − | |
| AdRL6 | pIX | − | MR1 | − | |
| AdRL34 | mSEAP | pIX | − | MR1 | − |
| AdRL28 | mSEAP | pIX | + | GFP | − |
| AdRL29 | mSEAP | pIX | + | GFP | + |
| AdRL31 | mSEAP | pIX | + | MR1 | − |
| AdRL32 | mSEAP | pIX | + | MR1 | + |
| AdRL39 | mSEAP | pIX | + | − | |
| AdRL71 | CMV | + | GFP | + | |
| AdRL74 | CMV | + | MR1 | + | |
| AdRP2687 | RFP | MLP | + | MR1 | + |
| AdRP2698 | RFP | MLP | + | GFP | + |
| AdRP2595 | mSEAP | pIX | − | AFAI | + |
| AdRP2823 | GFP | pIX | − | AFAI | + |
a
Promoter used to regulate expression of the pIX expression cassette.
b
Presence (+) or absence (−) of a endoplasmic reticulum signal peptide on the pIX expression cassette.
c
With the exception of AdRP2218 and AdAVH6, all viruses contain a FLAG epitope tag on the C terminus of pIX (located between pIX and GFP, MR1, or AFAI).
d
Presence (+) or absence (−) of a C-terminal nuclear localization sequence (NLS) on the pIX construct.