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. 2010 Jul 14;84(19):9889–9896. doi: 10.1128/JVI.00732-10

FIG. 2.

FIG. 2.

Unchanged steady-state levels of myosin Va protein in HSV-infected cells. Shown is an immunoblot showing levels of myosin Va in mock-infected and HSV-1(F)-infected HEp-2 total cell lysates. Cells were mock infected or infected with 5 PFU per cell HSV-1(F) and lysed in 2× denaturing buffer at 18 hpi, and proteins were separated on a polyacrylamide gel and transferred to nitrocellulose. The blot was probed with a goat anti-myoVa antibody and rabbit anti-ICP8 antibody. Immunoreactivity of goat anti-lamin B antibody served as a loading control.