FIG. 3.

A myosin Va epitope is exposed preferentially in HSV-infected cells. HeLa cells were mock infected or infected with HSV-1(F) (MOI = 5 PFU per cell). At 16 hpi the cells were washed in PBS, fixed in 3% PFA for 15 min, and permeabilized with 0.1% Triton X-100. Fixed cells were immunostained with rabbit anti-myosin Va (top rows) or goat anti-myosin Va (bottom row). Mouse antibody to ICP4 was used to mark infected cell nuclei. Red, myoVa with epitope in tail region; green, myoVa with epitope in N-terminal region; blue, ICP4.