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Antigenic characterization of purified trimeric SF162-derived gp140 constructs. (A) Binding curves of individual MAbs or IgG-CD4 to gp140 proteins. The epitope specificity of each MAb is shown in parentheses. MAbs and IgG-CD4 were tested at an initial concentration of 10 to 20 μg/ml against purified trimeric gp140 protein at 50 ng. Error bars indicate standard deviations. (B) The calculated half-maximal binding titers (μg/ml) are reported.
