TABLE 2.

Antibody titers and epitope specificities of immune plasma antibodies determined by ELISA

Group	Animal	Antibody binding fora:
		gp140b	N′ V2 (SF162)	C′ V2 (SF162)	V3 (SF162)	V1 (SF162)	V1 (YU2)	V1 (JRFL)	V1 (89.6)	V1 (HxB2)	gp41 (HxB2)	2F5	4E10	Scrambled V3
A (SF162/YU2 V1)	2071	69,894	—	—	5,879	—	—	—	—	—	21,778	—	—	—
	2072	39,831	—	—	9,974	—	—	—	—	—	6,309	—	—	—
	2073	19,183	—	—	5,209	—	—	—	—	—	4,043	—	—	—
	2074	12,486	—	—	6,511	—	—	—	—	—	2,885	—	—	—
	Avgc	35,348 (P = 0.0286)d	—	—	6,893 (P = 0.0286)	—	—	—	—	—	8,754 (P = 0.200)	—	—	—
B (SF162/JRFL V1)	2075	52,690	—	—	9,844	—	—	—	—	—	24,854	—	—	—
	2076	26,157	—	—	7,296	—	—	—	—	—	5,990	—	—	—
	2077	14,831	—	—	2,854	—	—	—	—	—	4,026	—	—	—
	2078	16,915	—	—	2,030	—	—	—	—	—	10,837	—	—	—
	Avg	27,648 (P = 0.0286)	—	—	5,506 (P = 0.0286)	—	—	—	—	—	11,427 (P = 0.3429)	—	—	—
C (SF162/89.6 V1)	2079	60,791	—	—	11,970	—	—	—	191	—	4,334	—	—	—
	2080	307,200	—	—	95,925	—	—	—	—	—	20,263	—	—	—
	2081	49,491	—	—	17,765	—	—	—	398	—	1,287	—	—	—
	2082	86,042	—	—	24,402	—	—	—	479	—	7,069	—	—	—
	Avg	125,881 (P = 0.3429)	—	—	37,515 (P = 0.3429)	—	—	—	356	—	8,238 (P = 0.200)	—	—	—
D (SF162/HxB2 V1)	2083	14,429	—	—	1,266	—	—	—	—	—	2,265	—	—	—
	2084	13,528	—	—	1,838	—	—	—	—	—	1,378	—	—	—
	2085	56,312	—	—	5,751	—	—	—	—	—	6,964	—	—	—
	2086	75,455	—	—	27,728	—	—	—	—	—	14,440	—	—	—
	Avg	39,931 (P = 0.0286)	—	—	9,146 (P = 0.0571)	—	—	—	—	—	6,261 (P = 0.200)	—	—	—
E (V1 scaffold cocktail)	2059	78,145	—	—	31,823	—	—	—	—	—	14,998	—	—	—
	2060	57,647	—	—	24,543	—	—	—	—	—	12,049	—	—	—
	2061	32,430	—	—	8,399	—	—	—	—	—	7,157	—	—	—
	2062	20,450	—	—	6,971	—	—	—	—	—	3,484	—	—	—
	Avg	47,168 (P = 0.0286)	—	—	17,934 (P = 0.1143)	—	—	—	—	—	9,422 (P = 0.0286)	—	—	—
F (ΔV1SF162)	1918	93,142	—	—	13,872	—	—	—	—	—	29,758	—	—	—
	1919	32,235	—	—	22,316	—	—	—	—	—	14,225	—	—	—
	1920	98,272	—	—	39,657	—	—	—	—	—	27,074	—	—	—
	1921	270,384	—	—	6,723	—	—	—	—	—	88,520	—	—	—
	Avg	123,508 (P = 0.0286)	—	—	20,642 (P = 0.200)	—	—	—	—	—	39,894 (P = 0.3429)	—	—	—
G (SF162 gp140)	1914	152,429	—	—	62,221	18,139	—	—	—	—	15,239	—	—	—
	1915	210,929	—	—	97,148	354	—	—	—	—	23,081	—	—	—
	1916	146,451	—	—	22,316	43,652	—	—	—	—	19,150	—	—	—
	1917	151,417	—	—	32,703	2,919	—	—	—	—	15,607	—	—	—
	Avg	165,307	—	—	53,597	16,266	—	—	—	—	18,269	—	—	—

^aValues indicate relative endpoint ELISA titers against the indicated recombinant proteins or linear peptides. Plasmas were collected at 2 weeks following the second gp140 protein immunization. Designations in parentheses indicate the viral isolate from which the peptide or protein is derived. —, reactivity not detected at the lowest plasma dilution tested (1:20).

^bSF162 and ΔV1SF162 immune plasmas were tested against SF162 and ΔV1SF162 gp140 trimeric proteins, respectively. Plasmas from groups A to D were tested against their matching SF162/EnvV1 gp140 protein. Group E animal plasma was tested against a mixture of each scaffold gp140 in equal parts.

^cAverage group values include only animals with detectable antibody titers.

^dStatistical P values, comparing binding titers for each group with those of group G (SF162 gp140).