TABLE 2.
Summary of nucleotide and predicted amino acid differences observed in single-genome amplification sequences
| Strain | Sequence no. | Mutation type (nucleotide position) | Effect on predicted polypeptide |
|---|---|---|---|
| Ken/07/80395 | Ken/07/80395/ii | Substitution (264) | None |
| Ken/07/80395/iii | Duplication (599-606) | Frameshift with a subsequent premature stop codon | |
| Ken/08/80900 | Ken/08/80900/ii | Deletion (792-851) | Reduction of length by 20 amino acids |
| Ken/08/80900/iii | Substitution (539) | Isoleucine-to-threonine change | |
| Ken/08/80767 | Ken/08/80767/ii | Substitution (592) | Immediate premature stop codon |
| Ken/08/80767/iii | Substitution (233) | Threonine-to-serine change | |
| Ken/08/80767/vi | Substitution (664) | Immediate premature stop codon | |
| Ken/08/80767/v | Deletion (599) | Frameshift with a subsequent premature stop codon | |
| Ken/08/80767/vi | Deletion (599) | Frameshift with a subsequent premature stop codon | |
| Deletion (792-851) | No effect due to earlier frameshift; otherwise this would cause a 20-amino-acid deletion | ||
| Ken/08/80767/vii | Deletion (836-939) | Frameshift, with no subsequent stop codon within the region sequenced |