FIG. 5.
The position of A47171-180 in the VACV immunodominance hierarchy. Groups of 3 C57BL/6 mice were infected with 106 PFU VACV WR i.p. (A) or i.d. (B) or with VACV MVA i.p. (C); 7 days later, splenocytes were used in ICS assays. Average percentages (and SEMs) of CD8+ T cells that produced IFN-γ in ex vivo stimulations with the indicated peptides are shown. Open bars show data for peptides from A47; the newly defined A47171-180 ([YAHINALEYI]) peptide is indicated with arrows. A4793 was not used for i.d. or MVA experiments, because preliminary data indicated that responses to this peptide were always at background levels after these immunizations. Results were confirmed by repeated experiments.