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. 2010 Jul 14;84(19):10016–10025. doi: 10.1128/JVI.00239-10

FIG. 3.

FIG. 3.

TMPRSS2 and TMPRSS4 mRNAs and TMPRSS2 protein are expressed in Caco-2 cells. (A) Analysis of TMPRSS2 and TMPRSS4 mRNA expression by RT-PCR. Total RNA was prepared from the indicated cell lines, treated with DNase, reverse transcribed, and used for the amplification of TMPRSS2, TMPRSS4, and GAPDH mRNAs by nested PCR. The results of single gels from which irrelevant lanes were removed are shown. (B) Analysis of TMPRSS2 and TMPRSS4 mRNA expression by quantitative RT-PCR. RNA was prepared as described for panel A, and TMPRSS2, TMPRSS4, and GUSB (housekeeping control) were amplified by TaqMan gene expression assays. Similar results were obtained in two independent experiments. No TMPRSS4 signal was detected in Vero E6 cells. (C and D) Expression of TMPRSS2 (C) or TMPRSS4 (D) in cell lines as determined by Western blotting. The indicated cell lines were lysed, and the expression of TMPRSS2 or TMPRSS4 was analyzed by Western blotting. Detection of β-actin served as a loading control. The results of a single gel from which irrelevant lanes were removed are shown in panel C. 293T + TMPRSS2, 293T cells transiently expressing TMPRSS2; 293T + TMPRSS4, 293T cells transiently expressing TMPRSS4.