FIG. 1.

Tegument-delivered pp71 is nuclear in differentiated but not undifferentiated cells. NHDFs (A, B, and C) and NT2 cells (D, E, F, and G) were plated on coverslips, infected at an MOI of 1 for 6 h, and then analyzed by indirect immunofluorescence for pp71, IE1, or pp65 and Oct4, as indicated. The boxed area of the merged panel of row D is shown as an enlarged image in row E for easier visualization of pp71 localization. In coculture experiments, NHDFs were transfected with either GFP and p14 (H) or GFP and an empty vector (I). Six hours later, they were overseeded with NT2 cells. These heterogenous cell cultures were fixed 15 h later and then analyzed by indirect immunofluorescence for Oct4. Nuclei were counterstained with Hoechst dye. NHDF nuclei in the second and fourth panels of row I are marked with asterisks.