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. 2010 Aug 4;84(19):9853–9863. doi: 10.1128/JVI.00500-10

FIG. 3.

FIG. 3.

pp71 localizes to the nuclei of NHDF-NT2 syncytia but not NT2-NT2 syncytia. NHDFs transfected with GFP and p14 (A and B) or GFP and an empty vector (C and D) were overseeded with infected NT2 cells (MOI = 5) and then fixed and analyzed for pp71 (A and C) or Oct4 (B and D) by indirect immunofluorescence. NT2 cells transfected with p14 and GFP (E and F) were overseeded with infected NT2 cells (MOI = 5) and then fixed and analyzed for pp71 (E) or Oct4 (F) expression by indirect immunofluorescence. Nuclei were counterstained with Hoechst dye. Asterisks in panel D indicate GFP-positive, Oct4-negative NHDF nuclei. Arrows in panel E and H point to punctate pp71. To allow for easier visualization of pp71, enlargements of the merged images from panels C (#) and D (##) are shown as panels G and H, respectively.