FIG. 5.

LF2 does not inhibit Rta activation of the BRLF1 or BZLF1 promoter. Reporter assay results from 293T cells transfected with luciferase reporter constructs from the BLRF1 (Rp) (i) or BZLF1 (Zp) promoter (ii) with or without Rta-V5 and LF2-HA are shown. Luciferase activities are shown as fold activation over reporter alone and were normalized for transfection efficiency as determined by β-galactosidase activity. Data are averages for six transfections from three independent experiments. Error bars indicate standard deviations. Western blots of the cell lysates with anti-Rta and anti-LF2 antibodies demonstrated Rta and LF2 protein expression levels.