FIG. 3.
Humoral responses in HI-infected/immunotherapy-treated and LI-infected/nontreated mice. Anti-FrCasE IgM (A) and IgG (B) responses developed in HI-infected/667 MAb-treated- and LI-infected/nontreated mice. Forty neonates per group of animals each were used per experiment as described in the legend to Fig. 1. Two independent experiments were conducted. (C) Anti-FrCasE neutralization activity in sera from HI-infected/immunotherapy-treated and LI-infected/nontreated mice. Neutralization activity was assayed using sera from 15-week-old mice. The presented data are the averages of values obtained from two groups of 15 mice analyzed in independent experiments. In the left panel, the assays of total neutralization activities in the sera from the various mice were conducted using identical volumes of sera collected from these mice. In the right panel, the assays of specific neutralization activities (neutralization activity per weight unit of anti-FrCasE immunoglobulin) were conducted after appropriate dilutions of serum samples so as to have the same amounts of anti-FrCasE immunoglobulins in all samples from HI-infected/immunotherapy-treated mice and from LI-infected/nontreated mice. Anti-FrCasE IgM (D) and total anti-FrCasE IgGs (E) responses developed in HI-infected/667 F(ab′)2-treated and HI-infected/672 MAb-treated mice. Forty neonates per group of animals each were used per experiment as described in the legend to Fig. 1. Two independent experiments were conducted. Error bars indicate standard deviations.