Skip to main content
. 2010 Jul 7;84(19):10169–10181. doi: 10.1128/JVI.00568-10

FIG. 6.

FIG. 6.

Characterization of the 672 MAb. (A) Identification of the Env epitope recognized by the 672 MAb. The SPOT analysis was carried out as described in Materials and Methods. Two hundred seventeen overlapping 15-mer amino acid peptides frameshifted by 3 residues and representing the complete CasBr Env were synthesized on a nitrocellulose membrane and probed with 672. Only the region with positive signals is presented. Amino acids are numbered with respect to the first amino acid of the surface protein (pep, peptide; aa, amino acid). (B) Localization of the epitope recognized by 672 in Env. The overall structure of CasBr Env is depicted. The epitope recognized by 672 (gray box) is contained in that recognized by 667 (white box). It is localized within the variable region A (VRA) of the receptor-binding domain (RBD). SS, signal sequence; SU, surface protein; TM, transmembrane protein.