FIG. 4.
Immunoblot analysis of wild-type HSV-1 and ΔICP27 virions purified from infected Vero or Vero 2-2 cells, respectively. Virions derived from Vero cells infected with wild-type HSV-1(F) (lane 1) or from Vero 2-2 cells infected with the ΔICP27 mutant virus (lane 2) at a multiplicity of infection of 5 PFU/cell and collected 24 h after infection were purified as described in Materials and Methods. Equal amounts of viral proteins were electrophoretically separated on a 10% denaturing polyacrylamide gel, transferred to a nitrocellulose sheet, and probed with rabbit polyclonal antibodies to VHS and UL38.